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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-β1 in wound healing
doi: 10.1186/s13287-020-01971-6
Figure Lengend Snippet: EPSC-Exos inhibited the expression of transforming growth factor-β1 (TGF-β1) and its downstream genes to suppress myofibroblast formation. a – d mRNA levels of TGF-β1, Smad2, α-SMA and collagen I in the healing tissue. e ESCs were co-cultured with HDF in Transwell plates (membrane well, 0.4 μm). IF and qRT-PCR were performed after culture for 48 h. f , g Expression levels of TGF-β1 of HDF in a co-cultured system using qRT-PCR and IF. Scale bar = 200 μm. *** P < 0.001; mean ± SD; n = 5. Ctrl, control; EGF, epidermal growth factor; EPSC-Exos, epidermal stem cell-derived exosomes; ESC, epidermal stem cell; HDF, human dermal fibroblast; TGF-β1, transforming growth factor β-1; α-SMA, α-smooth muscle actin; IF, immunofluorescence staining
Article Snippet: Next, the cells were incubated with
Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Derivative Assay, Immunofluorescence, Staining
Journal: Stem Cell Research & Therapy
Article Title: Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-β1 in wound healing
doi: 10.1186/s13287-020-01971-6
Figure Lengend Snippet: EPSC-Exos-specific microRNAs inhibited TGF-β1 expression of HDF. a Main miRNAs in EPSC-Exos using qRT-PCR. b A list of predicted binding sites of EPSC-Exos-specific miRNAs and their targets. c , d TGF-β1 mRNA levels of HDF treated with miR-425-5p agomir and miR-142-3p agomir of HDF, respectively. Scale bar = 200 μm. * P < 0.05; *** P < 0.001; mean ± SD; n = 5. Ctrl, control; EGF, epidermal growth factor; EPSC-Exos, epidermal stem cell-derived exosomes; HDF, human dermal fibroblast; TGF-β1, transforming growth factor β-1; IF, immunofluorescence staining
Article Snippet: Next, the cells were incubated with
Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Derivative Assay, Immunofluorescence, Staining
Journal: Stem Cell Research & Therapy
Article Title: Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-β1 in wound healing
doi: 10.1186/s13287-020-01971-6
Figure Lengend Snippet: EPSC-Exos promoted wound healing and reduced scarring. MiR-425-5p and miR-142-3p carried by EPSC-Exos might suppress myofiber and collagen I deposition via downregulating TGF-β1 expression
Article Snippet: Next, the cells were incubated with
Techniques: Expressing